The Alkaline Denaturation of DNA
نویسندگان
چکیده
منابع مشابه
The dynamics of DNA denaturation
Gaining knowledge and understanding of the structure and function of DNA, our genetic material, is crucial for dealing with diseases related to DNA. In 2004, the mapping of the complete human genome was accomplished, leading to an enormous progress in treating DNA-related diseases. The attention was for a long time directed at the DNA structure, specifically the sequence. However, the knowledge...
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The effect of heterogeneous sequence composition on the denaturation of double stranded DNA is investigated. The resulting pair-binding energy variation is found to have a negligible effect on the critical properties of the smooth second order melting transition in the simplest (Peyrard-Bishop) model. However, sequence heterogeneity is dramatically amplified upon adopting a more realistic treat...
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A new technique--partial denaturation of DNA in equilibrium CsCl density gradients at pH 11.4--is used to determine the distribution of intermediate states in the melting of mouse DNA. When the technique is applied in the preparative ultracentrifuge, the DNA is fractionated according to stability. Neutralization of the partially denatured fractions results in the recovery of most of the DNA in ...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1969
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(69)86452-0